ABSTRACT
Objective To built the reference range of peripheral blood T-lymphocyte subsets including CD3+ ,CD4+ ,CD8+ and CD4+ /CD8+ ratio in healthy adults of Ugyur and Han nationalities and to provide basis for the diagnosis ,therapy and prognosis of disease .Methods A total of 181 blood samples were collected from healthy adults .The cell chip quantitative detection technology was used to detect CD3+ ,CD4+ and CD8+ ,CD3+ ,CD4+ absolute value ,CD8+ and CD4+ /CD8+ ratio were compared between Ugyur and Han nationalities .Results CD8+ absolute counting and CD4+ /CD8+ ratio had no significant difference between Ugyur and Han nationalities(P>0 .05) ,while the CD3+ ,CD4+ absolute counting had significant difference(P<0 .05) .Conclusion The discrepancy of territory and living environment should be taken into account for building a reference values of CD4+ .
ABSTRACT
0.997 5);their average recovery rates were 99.93%,99.82%,100.02%,99.03%,and 98.79%(n=9),respectively,and their RSD were 0.66%,0.42%,0.55%,0.74%,and 0.98%(n=6),respectively.CONCLUSION:The established method is simple,rapid with accurate and reliable results thus applicable for the quality control of lecithin.